Officials said Satyendar Jain was summoned under the Prevention of Money Laundering Act (PMLA) and his statement will be recorded by the investigating officer of the case.
The PMLA case was registered by the Enforcement Directorate last year in August on the basis of a Central Bureau of Investigation (CBI) FIR.
The CBI complaint had said that the heath minister could not explain the source of funds received by four companies in which he was a shareholder.
It had registered a case against him, his wife and four others on charges of money laundering.
The CBI has questioned him in the case earlier.
CBI sources had said that Rs 4.63 crore was allegedly received through Prayas Info Solutions, Akinchan Developers, Managalyatan Projects and Indo-Metal Impex Pvt Limited in 2015-16.
They had said Satyendar Jain and his wife were allegedly holders of one-third of the shares in these companies during the period.
During CBI questioning, the minister and his wife could not explain the source of Rs 1.62 crore, approximately one-third of the funds received by the companies during 2015-17, they had said.
The probe agency had also claimed that these were shell firms used for parking money in the form of investments in equity shares in connivance with Kolkata-based shell companies.
"This apart, before becoming a public servant, he was allegedly involved in laundering Rs 11.78 crore during 2010-12 through these companies as well as other firms also based in New Delhi," the CBI had said.
The funds were allegedly used to purchase over 200 bighas of land in Auchandi, Bawana, Karala and Mohammed Mazvi villages in Delhi between 2010-16, CBI sources had said.
The Aam Aadmi Party (AAP), however, had refuted the CBI's allegations and said that reports of Jain's involvement in shell companies and benami land deals were "baseless".
Jain, also the minister for home, PWD, industries and urban development, is an MLA from the Shakurbasti constituency.
The ED probe will focus if he and other accused in the case created illegal assets by laundering the alleged tainted funds.
Get the latest election news, live updates and election schedule for Lok Sabha Elections 2019 on ndtv.com/elections. Like us on Facebook or follow us on Twitter and Instagram for updates from each of the 543 parliamentary seats for the 2019 Indian general elections. Election results will be out on May 23.