- Anshu Jamsenpa, 37, was blessed by the Dalai Lama before expedition
- Ms Jamsenpa has climbed Mount Everest five times
- Over 120 climbers have made it to Everest peak this season
Anshu Jamsenpa, 37, returned from the 8,848-metre (29,028-feet) peak last Tuesday, before turning around after a short rest to repeat the feat.
"Anshu reached the summit of Everest at 8:00 am (local time) today for the second time this season, setting a new record," said Dawa Lama of Dream Himalaya Adventures.
Ms Jamsenpa, a mother of two, was blessed by Tibetan spiritual leader the Dalai Lama before leaving for the expedition.
The current female record, certified by Guinness World Records, is held by Nepali climber Chhurim Sherpa, who in 2012 become the first woman to scale the peak twice in a season.
Ms Jamsenpa has climbed Mount Everest five times.
She intended to make the summit in 2014 but the climbing season was cancelled after an avalanche killed 16 Nepali guides.
Last week Nepali climber Lhakpa Sherpa broke her own record for the greatest number of summits by a woman after scaling the peak for an eighth time.
More than 120 climbers have made the summit this season, with the first ascents delayed by high winds, fresh snowfall and unusually cold temperatures.
There are concerns that a high number of summit permits issued by Nepal could cause dangerous "traffic jams" on the mountain as climbers target a brief window of favourable weather before the monsoon in June.
This year's climbing season has already been marred by two deaths, including legendary Swiss climber Ueli Steck who fell from a ridge during an acclimatization climb.