Mary Schafer, a conservator at The Nelson-Atkins Museum of Art, came across the tiny dried, brown carcass in the lower foreground while studying the painting of olive groves.
"I was mainly trying to understand the different layers of the painting and how it was constructed, and that's how I came upon part of the body of this little grasshopper," she told AFP.
"The fact that we have this little surprise of a grasshopper is a fun way to have a new look at a Van Gogh."
The find, announced this week, reflects the artist's practice of painting in the outdoors, where it was often windy enough to send dust, grass and insects flying.
"I must have picked up a good hundred flies and more off the four canvases that you'll be getting, not to mention dust and sand," van Gogh mused in an 1885 letter to his brother Theo.
"When one carries them across the heath and through hedgerows for a few hours, the odd branch or two scrapes across them."
But Van Gogh was no bug killer.
"Olive Trees" is part of a series of some 18 paintings Van Gogh completed on the subject in 1889 in Saint-Remy-de-Provence, France, where he had checked himself into an asylum. He died the following year.
Sand also was found in "Seascape near Les Saintes-Maries-de-la-Mer" (1888), a work he is believed to have painted from the beach on the Mediterranean. It is now held at the Van Gogh Museum in Amsterdam.
The Nelson-Atkins museum made its own find as part of an effort involving curators, conservators and outside scientists to take a fresh look at its collection of 104 French paintings and pastels using a variety of tools, including x-rays, ultraviolet light, the microscope and taking minuscule samples of the work.
Looking at a tiny slice of "Olive Trees," conservation scientist John Twilley determined that van Gogh had used a type of red lake pigment that faded over time due to light exposure.
(Except for the headline, this story has not been edited by NDTV staff and is published from a syndicated feed.) Click for more trending news
Get the latest election news, live updates and election schedule for Lok Sabha Elections 2019 on ndtv.com/elections. Like us on Facebook or follow us on Twitter and Instagram for updates from each of the 543 parliamentary seats for the 2019 Indian general elections.