Mr Badal, who is also president of the ruling Shiromani Akali Dal, made the allegation while addressing a 'Sadbhavna Rally' in Punjab town Nakodar, 40 km from Jalandhar city.
"The initial investigation by Punjab Police revealed that Pakistan's ISI was behind the recent incidents (of sacrilege of Guru Granth Sahib). Leaders of the Congress party were also behind the 'anti-national' forces which tried to disturb the hard-earned peace in the state," he said.
"We have handed over the investigation of this to the CBI. In the next 2-3 months, when the CBI report comes, you will come to know about the ISI and Congress role in these incidents," he said.
Mr Badal said that the anti-national forces were not only behind the incidents of desecration of Guru Granth Sahib, but also challenged the authority of sacred institutions of Sikhism - the Akal Takht and the Shiromani Gurdwara Parbandhak Committee - and violated the sanctity of Sarbat Khalsa.
Addressing the rally, Chief Minister Parkash Singh Badal accused of newly-appointed Congress state president Amarinder Singh of conniving with separatist Sikh leader Simranjit Singh Mann to divide the Sikh community and create disturbance in the state.
Get Breaking news, live coverage, and Latest News from India and around the world on NDTV.com. Catch all the Live TV action on NDTV 24x7 and NDTV India. Like us on Facebook or follow us on Twitter and Instagram for latest news and live news updates.