Mr Mishra has mentioned seven matters of 'corruption' including an 'exchange' of Rs two crore between Mr Kejriwal and Health minister Satyendar Jain.
"I have given statement on 7 serious issues of corruption by @ArvindKejriwal and his associates before Hon'ble Lokayukta," he said in a tweet.
The Lokayukta is mandated to enquire into corruption cases at state level.
However, Mr Mishra did not submit any evidence with Lokayukta Reva Khetrapal, saying he would do so later as some of the evidences were with his associate and 'independent investigator' Neil Haslam, who was out of town.
Mr Mishra said he would provide the date and the time of his visit to the chief minister's residence when Mr Jain allegedly gave Rs 2 crore to Mr Kejriwal in his presence after completion of the CBI investigation in the matter.
Latching on to the rebel AAP leader's statement, the Delhi unit of the BJP demanded that Mr Kejriwal step down as the chief minister on 'moral grounds'.
The matters of corruption raised by Mr Mishra in his statement included the water tanker 'scam', PWD 'scam' which involved Mr Kejriwal's relative late Surender Bansal, foreign trips by five AAP leaders and funds obtained by the party allegedly from 'shell' companies.
Mr Mishra was removed as Water Resources and Tourism minster from the Delhi cabinet on May 6 and had made the startling corruption allegation against Mr Kejriwal the next day.
A complaint of 'corruption' against Mr Kejriwal and Mr Jain was filed by the Delhi BJP's legal cell following Mr Mishra's allegation before the Lokayukta.
The Lokayukta had sent a notice to Mr Mishra for recording his statement in connection with his allegation.
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