With a key traders' body of Tamil Nadu too is backing the shutdown, retail outlets, grocery shops and establishments were closed in most of the places across the state, including Chennai and the Cauvery delta districts of Tiruchirappalli and Thanjavur.
The banks, pharmacies, hospitals and schools, however, remained open.
Due to the backing of key opposition-affiliated trade unions, including DMK's LPF and CPI's CITU, the operation of state-run buses, too was affected.
The shutdown for Cauvery Board was marked by protest demonstrations and impromptu rallies.
Tamil Nadu Congress Committee president Su Thirunavukkarasar and Viduthalai Chiruthaigal Katchi chief Thol Thirumavalavan too were accompanying Stalin.
In Tiruchirappalli, former minister and DMK strongman KN Nehru was leading a rally.
The DMK and other opposition parties have been staging protests across the state for the past four days over the issue.
Follow NDTV for latest election news and live coverage of assembly elections 2019 in Maharashtra and Haryana.
Subscribe to our YouTube channel, like us on Facebook or follow us on Twitter and Instagram for latest news and live news updates.