According to the final list posted on the website of the Tamil Nadu Chief Electoral Officer, a total of 72 nominations were accepted and out of it 13 nominees withdrew, leaving 59 candidates, including a woman, in the contest.
Election authorities notifying free symbols for independents announced 'pressure cooker' symbol for Mr Dhinakaran.
The seat has fallen vacant due to the death of sitting member and then chief minister Jayalalithaa on December 5 last year.
Out of the total 145 nomination papers received, as many as 73, including those of Jayalalithaa's niece Deepa Jayakumar and actor Vishal Krishna's had been rejected.
The first election to be held after the demise of the the AIADMK chief, the bypoll is seen as a litmus test for the ruling party which is facing a barrage of opposition accusations including alleged corruption.
The key contestants in the by-poll are ruling party's E Madusudanan, an old party warhorse and a former Minister, and DMK's up and coming leader N Marudhu Ganesh.
Rival AIADMK leader TTV Dhinakaran, touted as a formidable leader and having the following of party cadres by his camp, is fighting the by-poll as an independent.
BJP's Karu Nagararjan, a state-level functionary known for taking up the cudgels on behalf of his party in TV debates, is also seeking to test his fortunes.
Mr Dhinkaran had unsuccessfully moved the Delhi High Court seeking allotment of the 'hat' symbol, which was given to him in the rescinded April 12 bypoll to the constituency as the then candidate of V K Sasikala led faction.
Freezing the AIADMK's "two leaves" symbol after then rebel leader and now deputy chief minister O Panneerselvam staked claim to it, the Election Commission had then allotted free symbols to the two groups.
However, after the merger of factions led by chief minister K Palaniswami, which dumped Sasikala-Dhinakaran later, and Panneerselvam in August this year, the Election Commission alloted the 'two leaves' to them.
Counting of votes will be held on December 24 and the result is expected in a matter of few hours on that day.
RK Nagar has an electorate of 2,28,234 comprising 1,10,903 men, 1,17,232 women and 99 transgenders.
Jayalalithaa was elected from the constituency in May 2016 general election to the state assembly.
She had earlier also got elected from there in a by-poll.