External Affairs Ministry spokesperson Gopal Baglay said Mr Jadhav's well-being is a "matter of great concern" to India.
"We haven't seen, we haven't met (Mr Jadhav). He has been in Pakistan's custody for more than a year. So the well-being and state of health of Jadhav is a matter of great concern.
"We have asked Pakistani government earlier also, and yesterday, our High Commissioner (Gautam Bambawale) made a request on providing a report on his medical condition. So we await Pakistan's response," Mr Baglay told the Press Trust of India.
Mr Jadhav was arrested on March 3, 2016, allegedly from Balochistan. India says he was kidnapped in Iran, where he ran a small business.
Mr Jadhav's mother has filed an appeal at a higher court in Pakistan. The appeal was filed in the name of Avanti Jadhav by the Indian envoy in Islamabad in the Pakistan court of appeals yesterday.
In December 2016, Pakistan's foreign affairs advisor Sartaj Aziz had told the senate that the evidence against Mr Jadhav was not conclusive.