"The American vice president is not welcome in Palestine," Jibril Rajoub, a senior member of Palestinian president Mahmud Abbas's Fatah, told AFP.
Rajoub also signalled that Abbas would not meet with Pence during his planned visit later this month.
"And President Abbas will not welcome him because of the statements he made" about Jerusalem.
Abbas has not made similar comments and his office could not immediately be reached.
The White House warned Thursday that cancelling a planned meeting between Abbas and Pence in the wake of the US policy shift on Jerusalem would be "counterproductive".
The move was fully supported by Pence.
Abbas said Trump has disqualified the United States from its traditional role as peace broker in the Middle East conflict.
Pence is expected to visit Israel and the Palestinian territories sometime before Christmas.
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