In a statement, the Saudi hajj ministry said Qataris and residents of the Gulf emirate could join the pilgrimage as they were already "electronically registered for the hajj" and they had the necessary permits from Riyadh and Doha.
But the ministry has imposed restrictions on Qatari pilgrims arriving by plane, saying they must use airlines in agreement with the Saudi authorities.
They also needed to get visas on arrival in Jeddah or Medina, their sole points of entry in the kingdom, the ministry added.
Saudi Arabia and its allies Bahrain, Egypt and the United Arab Emirates cut diplomatic ties and imposed sanctions on Doha in June, including the closure of their airspace to Qatari airlines.
The four Arab states accuse Qatar of supporting extremists and of growing too close to Shiite-dominated Iran, the regional arch-rival of Sunni-ruled Saudi Arabia.