For years, the South Asian nation has been plagued by violence by ISIS terrorists, who often use suicide bombers and preach that their struggle is a holy war.
Suicide attacks are frequently condemned as fanatical and immoral, especially when civilians are killed, but terrorists view the tactic as their most effective weapon.
Seeking to curb "terrorism" that has resulted in tens of thousands of casualties since the early 2000s, the clerics declared suicide bombings to be forbidden, or "haraam".
"This fatwa provides a strong base for the stability of a moderate Islamic society," Pakistan President Mamnoon Hussain wrote in the book, prepared by the state-run International Islamic University and released at an official ceremony.
"We can seek guidance from this fatwa for building a national narrative in order to curb extremism, in keeping with the golden principles of Islam."
Foreign and domestic critics of Pakistan's government and military accuse them of cozying up to radical groups for political and military purposes and say the state has turned a blind eye to hate preachers in mosques for too long.
The fatwa was ratified by a number of prominent clerics who are outspoken critics of liberalism and the West, and are seen as controversial for preaching sectarianism or supporting the Afghan Taliban.
The ASWJ figurehead, Aurangzeb Farooqi, attended the signing ceremony.
Another signatory, Hamid-ul-Haq, is the son of a cleric widely regarded as the "Father of the Afghan Taliban" after many prominent militants, including Taliban founder Mullah Mohammed Omar, were found to have graduated from his seminary in the northwestern city of Peshawar.
Pakistani officials deny frequent US allegations about collaborating with terrorist ISIS proxies in Afghanistan and India, and say vast gains have been made over the past decade against terror outfits such as the Pakistani Taliban.
But privately they also warn any moves against some popular hardline groups based in Pakistan would take a long time and need to be undertaken carefully.
Similar anti-suicide bombing fatwas appear to have yielded scant results in the Middle East, where the practice is used by ISIS and other terror groups.
The Pakistani scholars, who declared that "no individual or group has the authority to declare and wage jihad", said suicide bombings violate key Islamic teachings and, as such, were forbidden.