The official, who spoke on condition of anonymity, declined to give any details about the duration of the questioning or what was learned.
American officials have many questions for them, such as, where other top Qaeda operatives are located; where Bin Laden lived before moving to Abbottabad, Pakistan; which Pakistani officials may have visited the compound in Abbottabad.
The wives however, are believed to have lived very cloistered lives, and it is unclear what they may know or how cooperative they may be.
The three widows, Khairiah Sabar, Siham Sabar and Amal Ahmed Abdulfattah, who is also known as Amal Ahmed al-Sadah, had been held and questioned for days by Pakistani officials before the CIA interrogators spoke to them. Ms Abdulfattah, who is Yemeni, was shot in the leg during the assault on Bin Laden's compound by Navy Seals.