Zimbabwean hunter Theo Bronkhorst has denied any wrongdoing and says his family and business have been destroyed in the wake of the lion's death, which triggered an international outcry.
The government last week said it would not charge Walter Palmer, the American dentist who used a bow and arrow to kill Cecil, a rare, black-manned lion outside Hwange National Park. It said Palmer's hunting papers were in order, which angered conservationists.
A Hwange magistrate dismissed Bronkhorst's application to have his indictment quashed, as well as his request to be allowed to ask the Constitutional Court to rule on whether his rights had been infringed upon.
His trial has been set for November 20.
Prosecutors say they will call five witnesses, including the owner of the game park where Cecil was killed.
The park's agency has said prosecutors also plan to present 13-year-old Cecil's head in court as evidence.
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