Speaking to US comedian John Oliver in Dharamsala, where the exiled Tibetan government is based, the Dalai Lama cast doubt on his reincarnation by saying he might be the last in line.
Foreign Ministry spokesman Geng Shuang noted that the interview appeared in an entertainment programme.
"The Dalai Lama's comments in the interview perhaps appeared humorous and funny, but these words are all lies that do not accord with the facts," Geng said.
"We often say that the 14th Dalai Lama is a political exile who wears religious clothing to engage in anti-China separatist activities," he added.
"Now it seems he is an actor, who is very good at performing, and very deceptively."
China brands the Nobel Peace laureate a dangerous separatist. The Dalai Lama, who fled into exile in India after a failed uprising against Chinese rule in 1959, denies espousing violence and says he only wants genuine autonomy for Tibet.
Tibetan Buddhism holds that the soul of a senior lama is reincarnated in the body of a child on his death.
China says the tradition must continue and it's officially atheist Communist leaders have the right to approve the Dalai Lama's successor, as a legacy inherited from China's emperors.
The Dalai Lama has suggested previously the title could end with him, when he dies. China accuses him of betraying, and being disrespectful of, the Tibetan religion, by saying there might be no future reincarnations.
(With inputs from Reuters)