Spread-betters also expected a shaky start for European shares, forecasting Britain's FTSE, Germany's DAX and France's CAC to open flat to slightly lower.
Japan's Nikkei lost more than one per cent as the yen firmed. Other decliners included Australian stocks, which fell 1.0 per cent.
Hong Kong's Hang Seng and South Korea's KOSPI stood little changed.
Shanghai see-sawed in and out of negative terrain, and was last up 0.1 per cent, after rallying on Tuesday on expectations MSCI could add China's mainland stocks to its emerging market benchmark for the first time.
David Dai, Shanghai-based investor director at Nanhai Fund Management Co, said any market rally was unlikely to be sustainable at this stage.
"The economy is still weak, and the Fed will likely raise rates soon. I don't think the market will go up much further. The best strategy now is to take profit."
There was little market reaction to the official and private surveys on China's manufacturing activity, which were roughly in line with expectations, suggesting the world's second-largest economy is still struggling to regain traction.
The Caixin/Markit Manufacturing Purchasing Managers' index (PMI) showed activity at China's factories shrank for a 15th straight month in May. The official manufacturing May PMI painted a slightly more optimistic picture and stood unchanged from the previous month at 50.1
MSCI's broadest index of Asia-Pacific shares outside Japan recovered from an earlier dip and crawled up 0.1 per cent.
The Dow shed 0.5 per cent and the S&P 500 dipped 0.1 per cent on Tuesday, as energy shares weakened in the wake of a slip in oil prices and offset a rise in safe-haven utilities.
Crude pulled back from eight-month highs reached last week amid expectations that a global glut was easing, falling overnight to profit taking. US crude was last down 0.7 per cent at $48.76 a barrel and Brent fell 0.8 per cent to $49.51.
The fall in oil prices was exacerbated after United Arab Emirates Oil Minister Suhail bin Mohammed al-Mazroui said he was happy with the oil market, noting that prices had been correcting higher.
The UAE oil minister's comments touched a nerve in a market wary that an OPEC (Organization of Petroleum Exporting Countries) meeting on Thursday may not pave the way for a production freeze.
"The political will of the OPEC countries to enact a production freeze is clearly waning. A production freeze is unlikely to come up as an agenda at the June meeting," wrote Yoshimasa Maruyama, chief market economist at SMBC Nikko Securities in Tokyo.
"A sense of crisis among the oil producing countries appears to have receded following the recent spike in crude oil prices."
In currencies, the dollar was down 0.6 per cent at 110.010 yen, having come off a one-month high of 111.455 struck on Monday after Federal Reserve Chair Janet Yellen's comments raised expectations for a near-term US rate hike.
The euro was at $1.1120, putting some distance between a two-and-a-half-month low of $1.1097 touched on Monday.
US data overnight saw personal income-related and housing indicators come in strong, while the Chicago manufacturing PMI and consumer confidence data proved disappointing.
The Australian dollar was up 0.6 per cent to $0.7277 helped by stronger-than-expected first quarter economic growth, which pushed it further away from last week's three-month low of $0.7145.
Sterling was on the defensive, last trading little changed at $1.4490 after dropping more than one percent on Tuesday, after polls showing those who support Brexit may be increasing.
© Thomson Reuters 2016