The agency questioned Mr Kalmadi for the second time in less than a month. He was questioned in a separate case of alleged forex violations on March 25 by the agency.
Mr Kalmadi has denied any wrongdoing in these cases.
The agency's probe into money laundering charge against the Pune Member of Parliament is with regard to its complaint on the basis of a First Information Report (FIR) by the Central Bureau of Investigation (CBI) on charges of cheating, conspiracy and corruption in the Timing, Scoring Result system (TSR) contract of the Commonwealth Games.
The CBI had arrested Mr Kalmadi last year in this case and he is now out on bail.
His role in awarding the contract for installing the Timing-Scoring-Result system to Swiss Times Omega at a huge cost of Rs 141 crore is under the ED's probe.
During questioning today, Mr Kalmadi was asked about the various clearances and remittances the CWG organising committee had made in awarding the TSR contract.
The agency had registered a case under the provisions of the Prevention of Money Laundering Act (PMLA) last year.
The ED is also probing Mr Kalmadi's role in the alleged forex-related contraventions in the conduct of the Queens Baton Relay (QBR) held in 2009 in London and it had questioned him in this case on March 25 last.